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What this bug is used for?

You may have encountered some of them under stones when being curious on rivers or lakes, but did you already know that they can be used to determine the quality of the water you are looking at?

With the adoption of the EU Water Framework Directive in 2000, European countries were bound to assess the Ecological Status of their water bodies (e.g. rivers, lakes) through hydromorphological, physicochemical and biological quality elements (see the video below for further information). This is where these little animals come into action, as macroinvertebrates (animals lacking a backbone that are easily seen without the use of a microscope) are one of the elements that can be evaluated to know the biological status of a given water body.

Source: EnvironmentAgencyTV

There are various reasons why the use of macroinvertebrates can sometimes be better than traditional water analysis:

  • they provide a time-integrated measure, because as living organisms they are sensitive to any past or present chemical, physical, hydrological and biological conditions of their ecosystem
  • they are easy to collect, transport and less prone to be contaminated
  • their analysis is relatively cheap and doesn’t require special equipment

Having said this, the next step for using these animals for water quality assessment purposes is to know how to collect and analyse them. This week I’m heading north for a field trip to Lake District and hopefully I’ll be back with some insights!

References:

  • Coelho JP, Lillebø AI, Pacheco M, Pereira ME, Pardal, MA, Duarte AC (2010) Biota analysis as a source of information on the state of aquatic environments. In: Namieśnik J and Szefer P (Eds) Analytical measurements in aquatic environments. Analytical Chemistry Series. CRC Press, Boca Raton. pp 103-120.
  • European Commission (2000) Water Framework Directive, Directive 2000/60/EC of 22 December 2000, establishing a framework for community action in the field of water policy. Official Journal of the European Communities L 327 , 22/12/2000 P. 0001 – 0073
  • Friedrich G, Chapman D, Beim A (1996) The use of biological material. In: Chapman D (Ed) Water quality assessments: a guide to use of biota, sediments and water in environmental monitoring. Second edition. UNESCO/WHO/UNEP. CRC Press.

Not everything that creeps is made out of gold

ussing macroinvertebrates is simply, cheap and fun…what are their drawbacks then?

Reference conditions has to be established for each river basin what makes the BWMP also variable and dependent on the specific conditions of the river.

WFD Indicators and Definition of the Ecological
Status of Rivers
R. Carballo · J. J. Cancela · G. Iglesias · A. Marín ·
X. X. Neira · T. S. Cuesta
Received: 18 June 2007 / Accepted: 20 November 2008 /
Published online: 9 December 2008

macroinvertebrates can also be used as biomonitors! quantitative analysis to assess the concentration of different micropollutants.

Are biotic indices sensitive to river toxicants? A comparison of metrics based on diatoms and macro-invertebrates

Author links open overlay panelS.BlancoabE.BécaresaaDepartment of Environmental Management and Biodiversity, University of Leon, Faculty of Biology, E-24071 León, SpainbThe Institute of the Environment, University of León, La Serna, 58, E-24007 León, Spain

Received 2 November 2009, Revised 18 January 2010, Accepted 28 January 2010, Available online 18 February 2010.

Go and explore!

criticism to kick sampling: Comparability of macroinvertebrate biomonitoring indices river health derived from semi-quantitative and quantitative methodologies (2017)

biogeographically restricted in application. BMWP modified for the Iberian Peninsula (IBMWP). Several European countries currently employ single metrics, even for official use. Examples include the IBMWP (Alba-Tercedor et al., 2004), which has been used in Spain; the IBGN (AFNOR, 1992) in France; the IBE (APAT-IRSA/CNR, 2004) in Italy; and the BBI (De Pauw & Vanhooren, 1883) in Belgium. Other countries like the UK and Australia have chosen to develop predictive methods to provide expected values for comparison with observed values, such as RIVPACS (Armitage et al., 1983; Wright et al., 1993) and AusRivAS (Smith et al., 1999).  ( Munné, A. & Prat, N. Hydrobiologia (2009) 628: 203. https://doi-org.ezproxy.nottingham.ac.uk/10.1007/s10750-009-9757-1 )

Indices defined to assess how organic pollution affects macroinvertebrates not all types of pollution

insect members of the biotic community may be absent for part of the year/seasonal affect (BOOK)

How macroinvertebrates are used to assess the quality of a river

Now that we know we can use invertebrates to evaluate the quality of a given river, we need to understand the methodology to do it. And, as a picture is worth a thousand words, I will you show you the whole process with pictures from my recent field trip.

Firstly, we collect the samples using a technique called timed kick sampling (Everall N.C. et al, 2017). It consists on kicking the substrate of the riverbed and letting the current transport the macroinvertebrates downstream into a kick net that is being held at right angles to the flow with the open end facing upstream, as you can see on the pictures below. The kicking should be undertaken for 60 seconds in 3 different habitats of the chosen watercourse (Note: previous work should be done to identify the different habitats of the river, e.g.: pools, rapids, etc.; as different invertebrates dwell different habitats and all should be covered).

Then, we place what have been caught on the net into a white tray filled with a little quantity of river water and one by one we pick the macroinvertebrates with tweezers.

Finally, we place the invertebrates into little pots to help us identify them later in the laboratory and we fill the pots with ethanol-sucrose for preservation.

As you have seen, this methodology is quite simply and the equipment required is low-priced and easy to find: a kick net, a tray, a pair of tweezers and a fair amount of little pots. The next step is to investigate how we can apply our findings!

References:

  • Everall N.C. et al. (2017) Comparability of macroinvertebrate biomonitoring indices of river health derived from semi-quantitative and quantitative methodologies. Ecological Indicators, vol. 78, pages 437-448.

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